General description
- Product name: Protein A ELISA Kit
- Detection method: colorimetric
- Example type: Cell culture supernatant
- Test type: Sandwich (quantitative)
- Sensitivity: 3.7pg/ml
- Range: 7.8 pg/ml – 500 pg/ml
- Rehearsal time: 1h 30m
- Test duration: one-step test
Product Description
The Protein A ELISA Kit (ab215542) is a single 90 min wash sandwich ELISA designed for the quantitative measurement of Protein A in the cell culture supernatant. It uses our patented SimpleStep ELISA technology. Quantify Protein A with a sensitivity of 3.7 pg/ml. SimpleStep ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA plates. This sandwich ELISA approach allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the simple steps ELISA protocol summary in the imaging section for more details. Our SimpleStep ELISA technology provides several benefits:
- Single wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and superior antibody reproducibility
- Fully validated in biological samples
- 96-well plate breakable into 12 x 8-well strips
A 384-well SimpleStep ELISA microplate (ab203359) is available for use as an alternative to the 96-well microplate provided with SimpleStep ELISA kits.
Notes
Protein A (Staphylococcus aureus Protein A) is a 42 kDa protein that is characterized by its ability to bind to the Fc portion of immunoglobulins. Abcam has not requested and does not intend to request REACH Authorization for customer uses of products containing substances on the European Authorization list (Annex XIV). It is the responsibility of our customers to verify the need to apply the REACH authorization and any other relevant authorization for the intended uses.
Platform: Precoated microplate (12 x 8-well strips)
Properties
$torage instructions
Store at +4°C. Consult the protocols.
Relevance
Protein A is a surface protein of S. aureus that binds IgG molecules through its Fc region. In serum, bacteria will bind to IgG molecules in the wrong orientation on their surface, making opsonization and phagocytosis difficult. S. aureus mutants lacking protein A are phagocytosed more efficiently in vitro, and mutants in infection models have decreased virulence. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology.
Cell localization: secreted; cellular wall.
Alternative names
- gG-binding protein A
- Immunoglobulin G binding protein A
- SPA
- Staphylococcal protein A