To Plate or to Simply Unfreeze

To Plate or to Merely Unfreeze, That Is the Query for Optimum Plasmid Extraction

Many molecular biology functions require quick plasmid DNA extraction, spurring a number of research on easy methods to velocity up the method. It’s often instructed in commonplace laboratory protocols to plate out frozen glycerol bacterial shares previous to micro organism incubation in liquid media and subsequent plasmid extraction, though the rationale for that is typically unexplained (apart from for the isolation of single colonies). Given the commonality and significance of this laboratory operation, such a follow is time-consuming and laborious.

To check the influence of this follow and the choice direct culturing methodology, we investigated the affiliation between bacterial cell mass and its potential affect on plasmid yields from the two strategies. Our outcomes confirmed no distinction with preplating for 7 out of eight plasmid constructs used within the examine, suggesting that direct glycerol restoration would not result in poorer plasmid yields. The findings assist the rationale for direct glycerol restoration for plasmid extraction, with out the necessity of an intermediate preplating step.

pOET1.1N_6xHis transfer plasmid
2001011	Oxford Expression Technologies	10 µg	EUR 148.18

pOET1.1C_6xHis transfer plasmid
2001012	Oxford Expression Technologies	10 µg	EUR 148.18

pOET2.1N/C_6xHis transfer plasmid
2001031	Oxford Expression Technologies	10 µg	EUR 148.18

pOET-2 transfer plasmid (10ug)
GWB-65CD85	GenWay Biotech	0.01 mg	Ask for price

pOET-1 transfer plasmid (10ug)
GWB-5A59EB	GenWay Biotech	0.01 mg	Ask for price

pOET-4 transfer plasmid (10ug)
GWB-282B50	GenWay Biotech	0.01 mg	Ask for price

pOET-3 transfer plasmid (10ug)
GWB-23143B	GenWay Biotech	0.01 mg	Ask for price

pOET 2 N/C_6xHis™ Transfer Vector
GWB-001031	GenWay Biotech	10 ug	Ask for price

GWB-5A59EB-10UG - pOET-1 transfer plasmid (10ug)
GWB-5A59EB-10UG	Aviva Systems Biology	10ug	EUR 287

pOET3 transfer plasmid
200104	Oxford Expression Technologies	10 µg	EUR 148.18

pOET4 transfer plasmid
200105	Oxford Expression Technologies	10 µg	EUR 148.18

pOET5.1 transfer plasmid
200106	Oxford Expression Technologies	10 µg	EUR 148.18

pOET8.VE2 transfer plasmid
200122	Oxford Expression Technologies	10 µg	EUR 353.43

pOET8.VE3 transfer plasmid
200123	Oxford Expression Technologies	10 µg	EUR 353.43

pOET9 SV40 transfer plasmid
200134	Oxford Expression Technologies	10 µg	EUR 199.33

pOET9 EF1α transfer plasmid
200131	Oxford Expression Technologies	10 µg	EUR 199.33

pOET9 CCAG transfer plasmid
200132	Oxford Expression Technologies	10 µg	EUR 199.33

pOET6 BacMAM transfer plasmid
200107	Oxford Expression Technologies	10 µg	EUR 148.18

pOET-5 Transfer Vector (10ug)
GWB-200106	GenWay Biotech	10 ug	Ask for price

6XHis azide
12628	AAT Bioquest	1mg	EUR 301
Description: 6XHis azide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry.

6XHis azide
12628-1mg	AAT Bioquest	1 mg	EUR 295

Description: 6XHis azide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry.

6XHis alkyne
12629-1mg	AAT Bioquest	1 mg	EUR 295

Description: 6XHis alkyne is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools through the well-known click chemistry.

Anti-6xHis [N144/14R] Plasmid
PVT50418	Nova Lifetech	2ug	EUR 280

6XHis maleimide
12626-1mg	AAT Bioquest	1 mg	EUR 295

Description: 6XHis maleimide is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools.

pOET Sequencing Primers
GWB-200100	GenWay Biotech	2 x 100 ul	Ask for price

pCDNA3.1-6XHis-UBD
PVT30908	Nova Lifetech	2ug	EUR 280

pCDNA3.1-6XHis-UFM1
PVT30910	Nova Lifetech	2ug	EUR 280

pCDNA3.1-6XHis-ISG15
PVT30913	Nova Lifetech	2ug	EUR 280

pCDNA3.4-6XHis-UBE1L
PVT30453	Nova Lifetech	2ug	EUR 280

pCDNA3.1-6XHis-UBE2I
PVT27974	Nova Lifetech	2ug	EUR 280

pCDNA3.4-6XHis-UBE2L6
PVT30272	Nova Lifetech	2ug	EUR 280

PLTP (Plasma Phospholipid Transfer Protein)
MBS613178-02mL	MyBiosource	0.2mL	EUR 650

PLTP (Plasma Phospholipid Transfer Protein)
MBS613178-5x02mL	MyBiosource	5x0.2mL	EUR 2765

6XHis Succinimidyl Ester
12624	AAT Bioquest	1mg	EUR 301
Description: 6XHis Succinimidyl Ester is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools.

6XHis Succinimidyl Ester
12624-1mg	AAT Bioquest	1 mg	EUR 295

Description: 6XHis Succinimidyl Ester is an excellent building block to make 6XHis conjugates for developing His tag detection probes and purification tools.

LCK with C-tGFP tag for Plasma memberane marking (10ug transfection-grade plasmid)
RC100017	Origene Technologies GmbH	10 µg	Ask for price

LCK with C-tRFP tag for Plasma memberane marking (10ug transfection-grade plasmid)
RC100049	Origene Technologies GmbH	10 µg	Ask for price

6xHis Conjugated Antibody
MBS9446006-01mLAF350	MyBiosource	0.1mL(AF350)	EUR 480

6xHis Conjugated Antibody
MBS9446006-01mLAF405	MyBiosource	0.1mL(AF405)	EUR 480

6xHis Conjugated Antibody
MBS9446006-01mLAF488	MyBiosource	0.1mL(AF488)	EUR 480

6xHis Conjugated Antibody
MBS9446006-01mLAF555	MyBiosource	0.1mL(AF555)	EUR 480

6xHis Conjugated Antibody
MBS9446006-01mLBiotin	MyBiosource	0.1mL(Biotin)	EUR 480

CCND1 with C-tGFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100009	Origene Technologies GmbH	10 µg	Ask for price

LAMP1 with C-tGFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100016	Origene Technologies GmbH	10 µg	Ask for price

CCND1 with C-tRFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100041	Origene Technologies GmbH	10 µg	Ask for price

LAMP1 with C-tRFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100048	Origene Technologies GmbH	10 µg	Ask for price

LAMP1 with C-mGFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100089	Origene Technologies GmbH	10 µg	Ask for price

CCND1 with C-mGFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100094	Origene Technologies GmbH	10 µg	Ask for price

GBA2 with C-mGFP tag for Microsome marking (10ug transfection-grade plasmid)
RC100099	Origene Technologies GmbH	10 µg	Ask for price

LAMP1 with C-mRFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100123	Origene Technologies GmbH	10 µg	Ask for price

CCND1 with C-mRFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100128	Origene Technologies GmbH	10 µg	Ask for price

GBA2 with C-mRFP tag for Microsome marking (10ug transfection-grade plasmid)
RC100133	Origene Technologies GmbH	10 µg	Ask for price

LAMP1 with C-mBFP tag for Lysosome marking (10ug transfection-grade plasmid)
RC100157	Origene Technologies GmbH	10 µg	Ask for price

CCND1 with C-mBFP tag for Nucleus marking (10ug transfection-grade plasmid)
RC100162	Origene Technologies GmbH	10 µg	Ask for price

GBA2 with C-mBFP tag for Microsome marking (10ug transfection-grade plasmid)
RC100167	Origene Technologies GmbH	10 µg	Ask for price

pET- NLS- Cas9- 6xHis
PVT10639	Lifescience Market	2 ug	EUR 361.2

BID with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100007	Origene Technologies GmbH	10 µg	Ask for price

BID with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100039	Origene Technologies GmbH	10 µg	Ask for price

BID with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100090	Origene Technologies GmbH	10 µg	Ask for price

BID with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100124	Origene Technologies GmbH	10 µg	Ask for price

BID with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100158	Origene Technologies GmbH	10 µg	Ask for price

PDHA1 with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100006	Origene Technologies GmbH	10 µg	Ask for price

PDHA1 with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100038	Origene Technologies GmbH	10 µg	Ask for price

ATG12 with C-mGFP tag for Auophagasome marking (10ug transfection-grade plasmid)
RC100066	Origene Technologies GmbH	10 µg	Ask for price

PDHA1 with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100091	Origene Technologies GmbH	10 µg	Ask for price

ATG12 with C-mRFP tag for Auophagasome marking (10ug transfection-grade plasmid)
RC100100	Origene Technologies GmbH	10 µg	Ask for price

PDHA1 with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100125	Origene Technologies GmbH	10 µg	Ask for price

ATG12 with C-mBFP tag for Auophagasome marking (10ug transfection-grade plasmid)
RC100134	Origene Technologies GmbH	10 µg	Ask for price

PDHA1 with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100159	Origene Technologies GmbH	10 µg	Ask for price

pCDNA3.1-NKD1(mut)-6XHis
PVT29019	Nova Lifetech	2ug	EUR 280

GAP43 with C-tGFP tag for Neuroal axis marking (10ug transfection-grade plasmid)
RC100013	Origene Technologies GmbH	10 µg	Ask for price

GAP43 with C-tRFP tag for Neuroal axis marking (10ug transfection-grade plasmid)
RC100045	Origene Technologies GmbH	10 µg	Ask for price

ATG12 with C-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100004	Origene Technologies GmbH	10 µg	Ask for price

MAPRE3 with C-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100019	Origene Technologies GmbH	10 µg	Ask for price

ATG12 with C-tRFP tag for Autophagosome marking (10ug transfection-grade plasmid)
RC100036	Origene Technologies GmbH	10 µg	Ask for price

MAPRE3 with C-tRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100051	Origene Technologies GmbH	10 µg	Ask for price

MAPRE3 with C-mGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100072	Origene Technologies GmbH	10 µg	Ask for price

MAPRE3 with C-mRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100106	Origene Technologies GmbH	10 µg	Ask for price

MAPRE3 with C-mBFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)
RC100140	Origene Technologies GmbH	10 µg	Ask for price

pET-(-30)GFP-9xGGS-Cre-6xHis Plasmid
PVT17107	Lifescience Market	2 ug	EUR 390

TGOLN with C-tGFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)
RC100029	Origene Technologies GmbH	10 µg	Ask for price

TGOLN with C-tRFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)
RC100061	Origene Technologies GmbH	10 µg	Ask for price

TGOLN with C-mGFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100088	Origene Technologies GmbH	10 µg	Ask for price

TGOLN with C-mRFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100122	Origene Technologies GmbH	10 µg	Ask for price

TGOLN with C-mBFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100156	Origene Technologies GmbH	10 µg	Ask for price

His-tag \| 6xHis (polyclonal)
AS19-4255	Agrisera AB	100 µg	EUR 312

B4GalT1 with C-tGFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)
RC100005	Origene Technologies GmbH	10 µg	Ask for price

B4GalT1 with C-tRFP tag for Golgi apparatus marking (10ug transfection-grade plasmid)
RC100037	Origene Technologies GmbH	10 µg	Ask for price

COX8A (MTS) with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100092	Origene Technologies GmbH	10 µg	Ask for price

COX8A (MTS) with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100126	Origene Technologies GmbH	10 µg	Ask for price

COX8A (MTS) with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)
RC100160	Origene Technologies GmbH	10 µg	Ask for price

ZYX with C-tGFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
RC100032	Origene Technologies GmbH	10 µg	Ask for price

ZYX with C-tRFP tag for Focal adherin fiber marking (10ug transfection-grade plasmid)
RC100064	Origene Technologies GmbH	10 µg	Ask for price

ZYX with C-mGFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100086	Origene Technologies GmbH	10 µg	Ask for price

ZYX with C-mRFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100120	Origene Technologies GmbH	10 µg	Ask for price

ZYX with C-mBFP tag for Focal Adherin Fiber marking (10ug transfection-grade plasmid)
RC100154	Origene Technologies GmbH	10 µg	Ask for price

Histidine (6xHis-tag) Antibody
MBS5680102-1mg	MyBiosource	1mg	EUR 515

Histidine (6xHis-tag) Antibody
MBS5680102-5x1mg	MyBiosource	5x1mg	EUR 2110

OAIA00006-1MG - 6xHIS Antibody
OAIA00006-1MG	Aviva Systems Biology	1.0mg	EUR 859

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free)
MBS633130-01mg	MyBiosource	0.1mg	EUR 630

PLTP (Plasma Phospholipid Transfer Protein) (Azide Free)
MBS633130-5x01mg	MyBiosource	5x0.1mg	EUR 2680

OAIA00003-100UG - 6xHIS Antibody
OAIA00003-100UG	Aviva Systems Biology	0.1mg	EUR 259

His-tag \| 6xHis (100 µg, Clone AD1,1,10)
AS15-2937	Agrisera AB	100 µg	EUR 443

B4GalT1 (GTS) with C-mGFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100087	Origene Technologies GmbH	10 µg	Ask for price

B4GalT1 (GTS) with C-mRFP tag for Golgi Apparatus marking (10ug transfection-grade plasmid)
RC100121	Origene Technologies GmbH	10 µg	Ask for price

A plasmid toolbox for managed gene expression throughout the Proteobacteria

Managed gene expression is key for the examine of gene operate and our capability to engineer micro organism. Nevertheless, there may be at present no easy-to-use genetics toolbox that allows managed gene expression in a variety of various species. To facilitate the event of genetics techniques in a quick, straightforward, and standardized method, we constructed and examined a plasmid meeting toolbox that may allow the identification of well-regulated promoters in lots of Proteobacteria and probably past. Every plasmid consists of 4 classes of genetic elements (i) the origin of replication, (ii) resistance marker, (iii) promoter-regulator and (iv) reporter.

The plasmids might be effectively assembled utilizing ligation-independent cloning, and any gene of curiosity might be simply inserted rather than the reporter. We examined this toolbox in 9 totally different Proteobacteria and recognized regulated promoters with over fifty-fold induction vary in eight of those micro organism. We additionally constructed variant libraries that enabled the identification of promoter-regulators with assorted expression ranges and elevated inducible fold change relative to the unique promoter. A number of over 50 plasmids, which include the entire toolbox’s genetic elements, can be found for neighborhood use and can allow straightforward development and testing of genetics techniques in each mannequin and non-model micro organism.

Genetic reprogramming of twine blood derived endothelial colony forming cells in direction of human induced pluripotent stem cells utilizing episomal plasmids

Goal: This examine aimed to isolate human umbilical twine blood derived endothelial colony forming cells (ECFCs) adopted by their integration free reprogramming in direction of induced pluripotent stem cells (iPSCs) and molecular characterization of each cell varieties utilizing multicolour flowcytometery and immunofluorescence respectively.

Strategies: The twine blood was collected from 37-39 weeks of gestational ages after C-section ex-utero from Dow College Hospital. The ECFCs remoted after ficoll primarily based separation of twine blood mononuclear cells (CBMNCs) which on emergence characterised by means of move cytometry and reprogrammed in direction of induced pluripotent stem cells (iPSCs) utilizing episomal vectors, the iPSCs have been characterised utilizing immunofluorescence. The examine was carried out at Stem Cells and Regenerative lab, Dow Analysis Institute of Biotechnology and Biomedical Sciences, Dow College of well being sciences OJHA campus. The examine time period was about one 12 months (October 2017-October 2018); examine design was in vitro experimental. The pattern dimension of the examine was n=3.

Outcomes: The remoted ECFCs have been evaluated utilizing flowcytometery which confirmed constructive expression for CD31, CD34, CD146 cell floor markers and damaging for CD90. The profitable reprogramming of ECFCs in direction of iPSCs was confirmed by immunofluorescence utilizing OCT-4 which is taken into account to be a grasp regulator of pluripotency.

Conclusions: To the most effective of our information this examine was the primary try to integration free reprogramming of twine blood derived endothelial colony forming cells in direction of induced pluripotent stem utilizing episomal plasmids. Cells which have been remoted from twine blood and people which have been reprogrammed each have potential therapeutic functions in regenerative medication.

Increasing the SiMPl Plasmid Toolbox for Use with Spectinomycin/Streptomycin

We not too long ago developed the SiMPl plasmid toolbox, which is constituted by pairs of plasmids, generically indicated as pSiMPl^x_N and pSiMPl^x_C, which might be stably maintained in Escherichia coli with a single antibiotic x. The strategy exploits the cut up intein gp41-1 to reconstitute the enzyme conferring resistance towards the antibiotic x, whereby every enzyme fragment is expressed from one of many plasmids within the pair. pSiMPl plasmids are at present obtainable to be used with ampicillin, kanamycin, chloramphenicol, hygromycin, and puromycin. Right here, we introduce one other pair to be used with spectinomycin/streptomycin, broadening the appliance spectrum of the SiMPl toolbox. To search out useful splice websites in aminoglycoside adenylyltransferase, we apply a streamlined technique wanting completely on the flexibility of native cysteine and serine residues, which we first validated splitting the enzymes conferring resistance towards ampicillin, kanamycin, chloramphenicol, and hygromycin. This technique might be used sooner or later to separate different enzymes conferring resistance towards antibiotics.

To Plate or to Merely Unfreeze, That Is the Query for Optimum Plasmid Extraction

Many molecular biology functions require quick plasmid DNA extraction, spurring a number of research on easy methods to velocity up the method. It’s often instructed in commonplace laboratory protocols to plate out frozen glycerol bacterial shares previous to micro organism incubation in liquid media and subsequent plasmid extraction, though the rationale for that is typically unexplained (apart from for the isolation of single colonies). Given the commonality and significance of this laboratory operation, such a follow is time-consuming and laborious.

To check the influence of this follow and the choice direct culturing methodology, we investigated the affiliation between bacterial cell mass and its potential affect on plasmid yields from the two strategies. Our outcomes confirmed no distinction with preplating for 7 out of eight plasmid constructs used within the examine, suggesting that direct glycerol restoration wouldn’t result in poorer plasmid yields. The findings assist the rationale for direct glycerol restoration for plasmid extraction, with out the necessity of an intermediate preplating step.

The Ferric Citrate Uptake System Encoded in a Novel bla _CTX-M-3– and bla _TEM-1-Harboring Conjugative Plasmid Contributes to the Virulence of Escherichia coli

Escherichia coli is one main reason behind bacterial infections and may horizontally purchase antimicrobial resistance and virulence genes by means of conjugation. As a result of conjugative plasmids can quickly unfold amongst micro organism of various species, the plasmids carrying each antimicrobial resistance and virulence genes might pose a vital menace to public well being. Due to this fact, the identification and characterization of those plasmids might facilitate a greater understanding of E. coli pathogenesis and the event of latest methods in opposition to E. coli infections. As a result of iron uptake capability is a possible virulence trait of micro organism, we screened for E. coli conjugative plasmids capable of confer each iron uptake capability and ampicillin resistance. The plasmid pEC41, which was derived from the bacteremia scientific isolate EC41, was recognized. EC41, which carried the fimHbla _CTX-M-3 was initially recognized. pEC41 carried bla _CTX-M-3 and bla _TEM-1. The ferric citrate uptake (fec) system was recognized in pEC41 and was answerable for conferring iron uptake capability.

The fec system contributes to the pathogenesis of EC41 in systemic infections however not in urinary tract infections (UTIs). Nevertheless, this technique promoted aggressive health of a cystitis-associated scientific isolate to colonize urinary tracts. Moreover, the distribution of the fec system was associated to E. coli isolates related to human bacteremia and UTIs. In abstract, the current examine recognized a novel conjugative plasmid, pEC41, which conferred each antimicrobial resistance and an additional iron uptake capability to E. coli. The iron uptake capability was encoded within the fec system and contributed to E. coli pathogenesis. This examine is the primary to indicate that the fec system is a virulence consider E. coli.

HiPer® Plasmid DNA Extraction Teaching K
HTBM002E-20PR	EWC Diagnostics	1 unit	EUR 55.13
Description: HiPer® Plasmid DNA Extraction Teaching K

HiPer® Plasmid DNA Extraction Teaching K
HTBM003E-20PR	EWC Diagnostics	1 unit	EUR 65.04
Description: HiPer® Plasmid DNA Extraction Teaching K

SpinCol Plasmid Miniprep Kit From Bacterial cells (Teaching)
52166	Sisco Laboratories	20 expt. Kit	EUR 42.76

Description: Part E

SpinCol Plasmid Miniprep Kit From Bacterial cells (Teaching)
52166-1	Sisco Laboratories	50 expt. Kit	EUR 60.64

Description: Part E

Plasmid DNA Isolation Solution I
10760028-1	Bio-WORLD	250 mL	EUR 38.41

Plasmid DNA Isolation Solution I
10760028-2	Bio-WORLD	500 mL	EUR 58.34

Plasmid DNA Isolation Solution II
10760029-1	Bio-WORLD	250 mL	EUR 38.41

Plasmid DNA Isolation Solution II
10760029-2	Bio-WORLD	500 mL	EUR 48.8

Plasmid DNA Isolation Solution III
10760030-1	Bio-WORLD	250 mL	EUR 36.14

Plasmid DNA Isolation Solution III
10760030-2	Bio-WORLD	500 mL	EUR 55.37

QC Buffer for Plasmid DNA Isolation
10760036-1	Bio-WORLD	250 mL	EUR 31.21

QC Buffer for Plasmid DNA Isolation
10760036-2	Bio-WORLD	500 mL	EUR 52.75

QF Buffer for Plasmid DNA Isolation
10760037-1	Bio-WORLD	250 mL	EUR 29.98

QF Buffer for Plasmid DNA Isolation
10760037-2	Bio-WORLD	500 mL	EUR 52.75

QF Buffer for Plasmid DNA Isolation
10760037-3	Bio-WORLD	1 L	EUR 95.47

QBT Buffer for Plasmid DNA Isolation
10760035-1	Bio-WORLD	250 mL	EUR 41.97

QBT Buffer for Plasmid DNA Isolation
10760035-2	Bio-WORLD	500 mL	EUR 60.26

QBT Buffer for Plasmid DNA Isolation
10760035-3	Bio-WORLD	1 L	EUR 89.33

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)
MBS412018-10Columns	MyBiosource	10Columns	EUR 160

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)
MBS412018-250Columns	MyBiosource	250Columns	EUR 835

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)
MBS412018-50Columns	MyBiosource	50Columns	EUR 295

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)
MBS412018-5x250Columns	MyBiosource	5x250Columns	EUR 3860

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)
MBS412019-1x96DeepWellPlate	MyBiosource	1x96DeepWellPlate	EUR 390

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)
MBS412019-24x96DeepWellPlate	MyBiosource	24x96DeepWellPlate	EUR 6690

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)
MBS412019-2x96DeepWellPlate	MyBiosource	2x96DeepWellPlate	EUR 675

nexttec Plasmid DNA Isolation Kit from Bacteria (E. coli)
MBS412019-4x96DeepWellPlate	MyBiosource	4x96DeepWellPlate	EUR 1230

Plasmid DNA Isolation Solutions I, II, III
10760027-1	Bio-WORLD	1 Kit	EUR 154.19

SDS PAGE Kit (Teaching)
83775	Sisco Laboratories	15 expt. Kit	EUR 85.44

Description: Part E

DNA Ligation Kit (Teaching)
95043	Sisco Laboratories	10 expt. Kit	EUR 37.63

Description: Part E

ExoPure? Isolation Kit (Serum, Plasma)
K1238-10	Biovision	each	EUR 836.4

ExoPure? Isolation Kit (Serum, Plasma)
K1238-2	Biovision	each	EUR 470.4

FITC Conjugation Kit (Teaching)
76941	Sisco Laboratories	5 expt. Kit	EUR 71.5

Description: Part E

HiPer® PCR Teaching Kit
HTBM016-10PR	EWC Diagnostics	1 unit	EUR 65.04
Description: HiPer® PCR Teaching Kit

Southern Blotting Kit (Teaching)
46037	Sisco Laboratories	5 expt. Kit	EUR 102.55

Description: Part E

FitAmp Plasma/Serum DNA Isolation Kit
P-1004	EpiGentek	Ask for price Ask for price Ask for price	100 Samples 50 Samples 100 Samples

ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma)
CEIK-004L-30T	Creative Bioarray	30 T	Ask for price

Exosome Isolation kit (for serum & plasma)
P101	101Bio	10 rxn	EUR 199

Exosome Isolation kit (for serum & plasma)
P101L	101Bio	40 rxn	EUR 769

Exosome Isolation kit (for serum & plasma)
P101S	101Bio	2 rxn	EUR 69

OrgFrontier? Plasma Membrane Isolation Kit
K414-10	Biovision	each	EUR 679.2

Restriction Digestion Kit (Teaching)
18450	Sisco Laboratories	10 expt. Kit	EUR 72.18

Description: Part E

Restriction Digestion Kit (Teaching)
18450-1	Sisco Laboratories	50 expt. Kit	EUR 140.95

Description: Part E

HiPer® Ligation Teaching Kit
HTBM019-20PR	EWC Diagnostics	1 unit	EUR 86.49
Description: HiPer® Ligation Teaching Kit

HiPer® Ligation Teaching Kit
HTBM019-5PR	EWC Diagnostics	1 unit	EUR 32.76
Description: HiPer® Ligation Teaching Kit

HiPer® RA Test Teaching Kit
HTI019-20PR	EWC Diagnostics	1 unit	EUR 15.98
Description: HiPer® RA Test Teaching Kit

HiPer® RPR Test Teaching kit
HTI020-20PR	EWC Diagnostics	1 unit	EUR 14.35
Description: HiPer® RPR Test Teaching kit

HiPer® RT-PCR Teaching Kit
HTBM024-5PR	EWC Diagnostics	1 unit	EUR 102.79
Description: HiPer® RT-PCR Teaching Kit

HiPer® DOT ELISA Teaching Kit
HTI015-15PR	EWC Diagnostics	1 unit	EUR 26.94
Description: HiPer® DOT ELISA Teaching Kit

HiPer® VDRL Test Teaching Kit
HTI021-10PR	EWC Diagnostics	1 unit	EUR 11.64
Description: HiPer® VDRL Test Teaching Kit

HiPer® Transduction Teaching Kit
HTM005E-20PR	EWC Diagnostics	1 unit	EUR 158.76
Description: HiPer® Transduction Teaching Kit

HiPer® Transduction Teaching Kit
HTM005E-5PR	EWC Diagnostics	1 unit	EUR 44.02
Description: HiPer® Transduction Teaching Kit

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-10T	Creative Bioarray	10 T	Ask for price

ExoQualiTM Exosome Isolation Kit (Plasma & Serum)
CEIK-006L-30T	Creative Bioarray	30 T	Ask for price

HiPer® SDS-PAGE Teaching Kit
HTP001-10PR	EWC Diagnostics	1 unit	EUR 48.37
Description: HiPer® SDS-PAGE Teaching Kit

HiPer® SDS-PAGE Teaching Kit
HTP001-20PR	EWC Diagnostics	1 unit	EUR 73.27
Description: HiPer® SDS-PAGE Teaching Kit

HiPer® Nested PCR Teaching Kit
HTBM043-10PR	EWC Diagnostics	1 unit	EUR 48.56
Description: HiPer® Nested PCR Teaching Kit

HiPer® Native PAGE Teaching Kit
HTP003-5PR	EWC Diagnostics	1 unit	EUR 119.46
Description: HiPer® Native PAGE Teaching Kit

HiPer® InstaDNA™ Teaching Kit
HTBM033-5PR	EWC Diagnostics	1 unit	EUR 44.72
Description: HiPer® InstaDNA™ Teaching Kit

HiPer® Transformation Teaching Kit
HTBM017E-10PR	EWC Diagnostics	1 unit	EUR 65.04
Description: HiPer® Transformation Teaching Kit

HiPer® Ezy MIC™ Teaching Kit
HTM006E-10PR	EWC Diagnostics	1 unit	EUR 72.46
Description: HiPer® Ezy MIC™ Teaching Kit

Plasma Membrane Isolation Kit, Catalog: PI-0104
PI-0104	Akrivis Bio	50 preparations	EUR 395

Description: To isolate intact plasma membrane from mammalian cells.

HiPer® Multiplex PCR Teaching Kit
HTBM023-5PR	EWC Diagnostics	1 unit	EUR 65.39
Description: HiPer® Multiplex PCR Teaching Kit

HIPER® GMO DETECTION TEACHING KIT
HTBM035-5PR	EWC Diagnostics	1 unit	EUR 32.76
Description: HIPER® GMO DETECTION TEACHING KIT

Bacterial Transformation Kit - 1 (Teaching)
64279	Sisco Laboratories	15 expt. Kit	EUR 78.77

Description: Part E

Bacterial Transformation Kit - 1 (Teaching)
64279-1	Sisco Laboratories	30 expt. Kit	EUR 111.53

Description: Part E

Agarose Gel Electrophoresis Kit (Teaching)
66602	Sisco Laboratories	15 expt. Kit	EUR 44.05

Description: Part E

HiPer® Blood Grouping Teaching Kit
HTI008-100PR	EWC Diagnostics	1 unit	EUR 13.7
Description: HiPer® Blood Grouping Teaching Kit

HiPer® Sandwich ELISA Teaching Kit
HTI014-4PR	EWC Diagnostics	1 unit	EUR 40.36
Description: HiPer® Sandwich ELISA Teaching Kit

HiPer® DNA Estimation Teaching Kit
HTBC006-20PR	EWC Diagnostics	1 unit	EUR 107.91
Description: HiPer® DNA Estimation Teaching Kit

HiPer® DNA Estimation Teaching Kit
HTBC006-5PR	EWC Diagnostics	1 unit	EUR 35.08
Description: HiPer® DNA Estimation Teaching Kit

HiPer® RNA Estimation Teaching Kit
HTBC007-20PR	EWC Diagnostics	1 unit	EUR 115.82
Description: HiPer® RNA Estimation Teaching Kit

HiPer® RNA Estimation Teaching Kit
HTBC007-5PR	EWC Diagnostics	1 unit	EUR 37.59
Description: HiPer® RNA Estimation Teaching Kit

HiPer® Phage Titration Teaching Kit
HTM003E-10PR	EWC Diagnostics	1 unit	EUR 84.26
Description: HiPer® Phage Titration Teaching Kit

HiPer® PCR Application Teaching Kit
HTBM030-20PR	EWC Diagnostics	1 unit	EUR 275.37
Description: HiPer® PCR Application Teaching Kit

HiPer® PCR Application Teaching Kit
HTBM030-5PR	EWC Diagnostics	1 unit	EUR 79.73
Description: HiPer® PCR Application Teaching Kit

HiPer® Immunoprecipitation Teaching Kit
HTI016-5PR	EWC Diagnostics	1 unit	EUR 75.78
Description: HiPer® Immunoprecipitation Teaching Kit

HiPer® Western Blotting Teaching Kit
HTI009-20PR	EWC Diagnostics	1 unit	EUR 274.64
Description: HiPer® Western Blotting Teaching Kit

HiPer® Western Blotting Teaching Kit
HTI009-5PR	EWC Diagnostics	1 unit	EUR 82.63
Description: HiPer® Western Blotting Teaching Kit

HiPer® IgG Purification Teaching Kit
HTI022-5PR	EWC Diagnostics	1 unit	EUR 219.05
Description: HiPer® IgG Purification Teaching Kit

HiPer® GST Purification Teaching Kit
HTP004-5PR	EWC Diagnostics	1 unit	EUR 156.09
Description: HiPer® GST Purification Teaching Kit

HiPer® Immunohistochemistry Teaching Kit
HTI023-5PR	EWC Diagnostics	1 unit	EUR 144.14
Description: HiPer® Immunohistochemistry Teaching Kit

HiPer® Southern Blotting Teaching Kit
HTBM027-5PR	EWC Diagnostics	1 unit	EUR 100.09
Description: HiPer® Southern Blotting Teaching Kit

HiPer® Northern Blotting Teaching Kit
HTBM028-5PR	EWC Diagnostics	1 unit	EUR 130.55
Description: HiPer® Northern Blotting Teaching Kit

HiPer® Real-Time PCR Teaching Kit
HTBM032-10PR	EWC Diagnostics	1 unit	EUR 101.32
Description: HiPer® Real-Time PCR Teaching Kit

HiPer® Protein Estimation Teaching Kit
HTBC004-25PR	EWC Diagnostics	1 unit	EUR 94.6
Description: HiPer® Protein Estimation Teaching Kit

HiPer® Protein Estimation Teaching Kit (
HTBC005-5PR	EWC Diagnostics	1 unit	EUR 27.88
Description: HiPer® Protein Estimation Teaching Kit (

HiPer® Latex Agglutination Teaching Kit
HTI001-20PR	EWC Diagnostics	1 unit	EUR 59.93
Description: HiPer® Latex Agglutination Teaching Kit

HiPer® Restriction Mapping Teaching Kit
HTBM021-20PR	EWC Diagnostics	1 unit	EUR 96.52
Description: HiPer® Restriction Mapping Teaching Kit

HiPer® Restriction Mapping Teaching Kit
HTBM021-5PR	EWC Diagnostics	1 unit	EUR 46.1
Description: HiPer® Restriction Mapping Teaching Kit

ExoQuick ULTRA EV Isolation Kit for Serum and Plasma
EQULTRA-20A-1	SBI	20 reactions	EUR 941

HiPer® Teaching Kits-Handbook
MBTL001-1NO	EWC Diagnostics	1 unit	EUR 11.97
Description: HiPer® Teaching Kits-Handbook

BioLit Gene Amplification Kit - PCR (Teaching)
19317	Sisco Laboratories	10 expt. Kit	EUR 104.17

Description: Part E

BioLit Gene Amplification Kit - PCR (Teaching)
19317-1	Sisco Laboratories	25 expt. Kit	EUR 147.88

Description: Part E

HiPer® Yeast Transformation Teaching Kit
HTBM025E-10PR	EWC Diagnostics	1 unit	EUR 62.08
Description: HiPer® Yeast Transformation Teaching Kit

HiPer® Gel Extraction Teaching Kit (Colu
HTBM010-10PR	EWC Diagnostics	1 unit	EUR 48.37
Description: HiPer® Gel Extraction Teaching Kit (Colu

HiPer® Gel Extraction Teaching Kit (Solu
HTBM011-10PR	EWC Diagnostics	1 unit	EUR 58.31
Description: HiPer® Gel Extraction Teaching Kit (Solu

HiPer® Bacterial RNA Teaching Kit (Colum
HTBM042-10PR	EWC Diagnostics	1 unit	EUR 32.63
Description: HiPer® Bacterial RNA Teaching Kit (Colum

HiPer® Total RNA Extraction Teaching Kit
HTBM012-10PR	EWC Diagnostics	1 unit	EUR 58.31
Description: HiPer® Total RNA Extraction Teaching Kit

HiPer® Cloning Teaching Kit (Blue-White
HTBM036-5PR	EWC Diagnostics	1 unit	EUR 83.75
Description: HiPer® Cloning Teaching Kit (Blue-White

HiPer® Widal Test Teaching Kit (Slide Te
HTI017-20PR	EWC Diagnostics	1 unit	EUR 18.66
Description: HiPer® Widal Test Teaching Kit (Slide Te

HiPer® Widal Test Teaching Kit (Tube Tes
HTI018-10PR	EWC Diagnostics	1 unit	EUR 57.61
Description: HiPer® Widal Test Teaching Kit (Tube Tes

Minute TM Plasma Membrane Protein Isolation Kit (50 Tests)
SM-005	Inventbiotech	each	EUR 555

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)
P101NGS	101Bio	-	Ask for price

Genomic DNA Extraction (from leaves) Kit (Teaching)
61498	Sisco Laboratories	15 expt. Kit	EUR 89.29

Description: Part E

Genomic DNA Extraction (from bacteria) Kit (Teaching)
83462	Sisco Laboratories	15 expt. Kit	EUR 63.97

Description: Part E

miRNA Isolation Kit
FYG304-100P	Yeastern Biotech	100 Preps	Ask for price

miRNA Isolation Kit
FAMIK--001	Favorgen	Rxns: 100	EUR 398.4
Description: Contents of the kit: Rxns:100Lysis Buffer-10 ml Acid-Phenol-10 ml RNA Column and Collection Tube-100 sets Wash Buffer-10 ml Release Buffer-0.5 ml

miRNA Isolation Kit
FAMIK--002	Favorgen	Rxns: 50	EUR 267.6

Plant/Fungi DNA Isolation Kit
10760114-1	Bio-WORLD	50 Prep(s)	EUR 451.21

Plant/Fungi DNA Isolation Kit
26200	Norgen Biotek Corp	50 Preps	EUR 149.4

Plant/Fungi DNA Isolation Kit
26240	Norgen Biotek Corp	4 Preps	EUR 12

Plant/Fungi DNA Isolation Kit
26250	Norgen Biotek Corp	250 Preps	EUR 671.4

Total RNA Isolation Kit (Plant)
PDC07-0100	Bio-Helix	100 rxns	EUR 209

Total RNA Isolation Kit (Plant)
P-9104	EpiGentek	50 samples	EUR 416.7

Rapid Plant RNA Isolation Kit
PT4191	Bio Basic	50Preps	EUR 95.63

Exosome Isolation Kit
E601E001	EnoGene	20 tests	EUR 2500

Exosome Isolation Kit
EIK01	Creative BioMart	2T	EUR 800

Description: Chromatography Reagents

Exosome Isolation Kit
MBS8579443-20Tests	MyBiosource	20Tests	EUR 2625

Exosome Isolation Kit
MBS8579443-5x20Tests	MyBiosource	5x20Tests	EUR 12240

VEX Exosome Isolation Reagent (from plasma)
R603	Vazyme	10 ml	EUR 769.2

Endosome Isolation Kit
P528	101Bio	-	Ask for price

Genomic DNA Isolation Kit (Plant)
PDC10-0100	Bio-Helix	100 rxns	EUR 132

Microsome Isolation Kit
K249-50	Biovision	each	EUR 633.6

XCF COMPLETE Exosome and cfDNA Isolation Kit (for Serum and Plasma)
XCF100A-1	SBI	20 rxn	EUR 583

Genoirse Plant DNA Isolation Kit
GR101083	Genorise Scientific	50	EUR 223

HiPer® Relative Quantification Teaching
HTBM039-10PR	EWC Diagnostics	1 unit	EUR 80.9
Description: HiPer® Relative Quantification Teaching

HiPer® Absolute Quantification Teaching
HTBM040-10PR	EWC Diagnostics	1 unit	EUR 47.8
Description: HiPer® Absolute Quantification Teaching

HiPer® Affinity Chromatography Teaching
HTC002-5PR	EWC Diagnostics	1 unit	EUR 82.82
Description: HiPer® Affinity Chromatography Teaching

Protoplast Isolation Kit
30210002-1	Bio-WORLD	20 Prep(s)	EUR 272.26

Protoplast Isolation Kit
30210002-2	Bio-WORLD	50 Prep(s)	EUR 564.44

Minute TM Plasma Membrane Protein Isolation Kit for Plants (50 Tests)
SM-005-p	Inventbiotech	each	EUR 555

Chloroplast Isolation Kit
30210000-1	Bio-WORLD	20 Prep(s)	EUR 336

To Plate or to Merely Unfreeze, That Is the Query for Optimum Plasmid Extraction

pOET1.1N_6xHis transfer plasmid

pOET1.1C_6xHis transfer plasmid

pOET2.1N/C_6xHis transfer plasmid

pOET-2 transfer plasmid (10ug)

pOET-1 transfer plasmid (10ug)

pOET-4 transfer plasmid (10ug)

pOET-3 transfer plasmid (10ug)

pOET 2 N/C_6xHis™ Transfer Vector

GWB-5A59EB-10UG - pOET-1 transfer plasmid (10ug)

pOET3 transfer plasmid

pOET4 transfer plasmid

pOET5.1 transfer plasmid

pOET8.VE2 transfer plasmid

pOET8.VE3 transfer plasmid

pOET9 SV40 transfer plasmid

pOET9 EF1α transfer plasmid

pOET9 CCAG transfer plasmid

pOET6 BacMAM transfer plasmid

pOET-5 Transfer Vector (10ug)

6XHis azide

6XHis azide

6XHis alkyne

Anti-6xHis [N144/14R] Plasmid

6XHis maleimide

pOET Sequencing Primers

pCDNA3.1-6XHis-UBD

pCDNA3.1-6XHis-UFM1

pCDNA3.1-6XHis-ISG15

pCDNA3.4-6XHis-UBE1L

pCDNA3.1-6XHis-UBE2I

pCDNA3.4-6XHis-UBE2L6

PLTP (Plasma Phospholipid Transfer Protein)

PLTP (Plasma Phospholipid Transfer Protein)

6XHis Succinimidyl Ester

6XHis Succinimidyl Ester

LCK with C-tGFP tag for Plasma memberane marking (10ug transfection-grade plasmid)

LCK with C-tRFP tag for Plasma memberane marking (10ug transfection-grade plasmid)

6xHis Conjugated Antibody

6xHis Conjugated Antibody

6xHis Conjugated Antibody

6xHis Conjugated Antibody

6xHis Conjugated Antibody

CCND1 with C-tGFP tag for Nucleus marking (10ug transfection-grade plasmid)

LAMP1 with C-tGFP tag for Lysosome marking (10ug transfection-grade plasmid)

CCND1 with C-tRFP tag for Nucleus marking (10ug transfection-grade plasmid)

LAMP1 with C-tRFP tag for Lysosome marking (10ug transfection-grade plasmid)

LAMP1 with C-mGFP tag for Lysosome marking (10ug transfection-grade plasmid)

CCND1 with C-mGFP tag for Nucleus marking (10ug transfection-grade plasmid)

GBA2 with C-mGFP tag for Microsome marking (10ug transfection-grade plasmid)

LAMP1 with C-mRFP tag for Lysosome marking (10ug transfection-grade plasmid)

CCND1 with C-mRFP tag for Nucleus marking (10ug transfection-grade plasmid)

GBA2 with C-mRFP tag for Microsome marking (10ug transfection-grade plasmid)

LAMP1 with C-mBFP tag for Lysosome marking (10ug transfection-grade plasmid)

CCND1 with C-mBFP tag for Nucleus marking (10ug transfection-grade plasmid)

GBA2 with C-mBFP tag for Microsome marking (10ug transfection-grade plasmid)

pET- NLS- Cas9- 6xHis

BID with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid)

BID with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid)

BID with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)

BID with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)

BID with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)

PDHA1 with C-tGFP tag for Mitochondria marking (10ug transfection-grade plasmid)

PDHA1 with C-tRFP tag for Mitochondria marking (10ug transfection-grade plasmid)

ATG12 with C-mGFP tag for Auophagasome marking (10ug transfection-grade plasmid)

PDHA1 with C-mGFP tag for Mitochondria marking (10ug transfection-grade plasmid)

ATG12 with C-mRFP tag for Auophagasome marking (10ug transfection-grade plasmid)

PDHA1 with C-mRFP tag for Mitochondria marking (10ug transfection-grade plasmid)

ATG12 with C-mBFP tag for Auophagasome marking (10ug transfection-grade plasmid)

PDHA1 with C-mBFP tag for Mitochondria marking (10ug transfection-grade plasmid)

pCDNA3.1-NKD1(mut)-6XHis

GAP43 with C-tGFP tag for Neuroal axis marking (10ug transfection-grade plasmid)

GAP43 with C-tRFP tag for Neuroal axis marking (10ug transfection-grade plasmid)

ATG12 with C-tGFP tag for Autophagosome marking (10ug transfection-grade plasmid)

MAPRE3 with C-tGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

ATG12 with C-tRFP tag for Autophagosome marking (10ug transfection-grade plasmid)

MAPRE3 with C-tRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

MAPRE3 with C-mGFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

MAPRE3 with C-mRFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

MAPRE3 with C-mBFP tag for Cytoskeleton marking (10ug transfection-grade plasmid)

The Ferric Citrate Uptake System Encoded in a Novel bla _CTX-M-3– and bla _TEM-1-Harboring Conjugative Plasmid Contributes to the Virulence of Escherichia coli